Johnny Test (2005)

S2E19 Johnny X Strikes Back

Five notorious villains—Wacko, the Bee Keeper, Brain Freezer, Mr. Mittens, and Albert the butler—who have all faced defeat at the hands of Johnny, join forces to abduct him. With Johnny in peril, it's up to Susan, Mary, Dukey, Bling-Bling, Gil, and Bumper to come together and rescue him from their clutches.